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IDDRC Core-B
Co-Directors: Michael Brenner, Ph.D. and Anne Theibert, Ph.D.

Useful Lab Protocols For Researchers

Please contact our co-directors if you need additional resources at UAB.

The PDF contains information on the following lab protocols:

Competent cell

  • Making electro competent cells
  • Transforming electro competent cells
  • Making chemical competent cells
  • Transforming chemical competent

Primary cell culture

  • Neuronal culture
  • Astroglial culture
  • Glass coverslip preparation
  • Neuronal culture supplies

Primary cell transfections

Calcium-Phosphate Transfection

  • Calcium-Phosphate Transfection of Rat Hippocampal Neurons-Yuying
  • Calcium-Phosphate Transfection of Rat Hippocampal Neurons-Terri
  • Calcium-Phosphate Transfection of Rat Hippocampal slice-Terri

Amaxa Nucleofection

  • Basic Nucleofection Protocol for Primary Mammalian Neural Cells
  • Optimized Protocol for Primary Mouse Hippocampal Neurons
  • Optimized Protocol for Primary Mouse Astrocytes
  • Optimized Protocol for Primary Rat Astrocytes
  • Optimized Protocol for Primary Rat Hippocampal or Cortical Neurons
  • Optimized Protocol for Primary Rat Dorsal Root Ganglion Neurons

Oocyte Culture

  • Oocyte Preparation
  • Oocyte Injection
  • Oocyte Recipes

Useful links:
These links will go to sites we do not manage. Links are provided for information purposes only.

UAB Neurosciencecore Molecular Detection Core Protocol site

Current Protocols in Molecular Biology (CPMB):

http://www.uab.edu/lister/news/index.php?newsID=1&ID=94

NCBI Home:

http://www.ncbi.nlm.nih.gov/

NCBI Clone Registry:

http://www.ncbi.nlm.nih.gov/genome/clone/

UCSC Genome Bioinformatics:

http://genome.ucsc.edu/

GCG at UAB:

http://genome.microbio.uab.edu/GCG/GCGatUAB.htm

Promega Restriction Enzymes Resource: 

Promega International site

NEB Cutter:

http://tools.neb.com/NEBcutter2/index.php

 

Additional protocols will soon be placed on this site.