Brenner, Ph.D. and Anne
Technical Director: Scott
Phillips, Ph.D. Core is
located is SHEL 960, 962, and 972. Phone 205-975-2333.
The objective of the Recombinant
Technologies Core is to provide technical and
material support for molecular and cellular research by
IDDRC investigators at UAB. The Core combines support for
cutting edge procedures with outreach to the molecularly
naive to encourage and enable application of recombinant
techniques to their research projects. It provides state-of-the-art
equipment, dedicated technical expertise, comprehensive
hands-on training, and reagents in such areas as PCR, neuronal
culture, cell transfection, sequence database searching
and analysis, and recombinant DNA techniques. Through these
services, the Core facilitates studies at the molecular
level of brain development and function and disease processes.
It also promotes collaborative projects among IDDRC investigators,
and enables investigators to combine newly acquired cellular
and molecular tools with other approaches, such as electrophysiology
and behavior. The success of the Core in meeting the needs
of the IDDRC community is indicated by 2,476 logged uses
in the past year.
the past year the Core has logged 2,476 visits from 29 different
laboratories. Many of these were for routine use of equipment
such as the electroporator, gel documentation system, or tissue
culture facilities for working with the neuronal cultures. However,
a critical component of the Core has been the instruction and
training of students, staff and faculty. Molecular biology techniques
taught included DNA purification, PCR, gel imaging, subcloning,
nucleic acid database analysis, electroporation, neuronal transfection,
DNA and protein electrophoresis and western blotting, and cell
culture methods. During the past year alone, 47 individuals from
16 different labs were trained, including 22 graduate students,
9 postdoctoral fellows, 13 research staff and 3 faculty.
services are available without charge to all IDDRC investigators
and their staffs, except users are expected to provide supplies
in certain instances (e.g., rats for neuronal cell preparations).
Although IDDRC investigators have priority, other investigators
at UAB are encouraged to take advantage of the core facilities
as well. In most cases, these services will also be provided
without charge, although in certain instances there may be
a modest fee.
One of the important functions of the Recombinant Technologies Core is to provide
a skilled molecular and cellular biology technical staff. The technical director
is Dr. Scott Phillips. Scott has over 12 years of expertise in the area of
molecular and cellular neurobiology. In 2005 Scott received his Ph.D. from
the University of North Carolina at Chapel Hill in Cell and Developmental Biology
under the mentorship of Dr. Vytas Bankaitis. Scott then was a postdoctoral
fellow in the lab of Dr. Kendal Broadie at Vanderbilt University until 2010
where he received an NRSA postdoctoral fellowship for studying the neurodegenerative
Niemann-Pick type C disease. Scott's responsibilities include day-to-day administration
of the Core and supervision of the research technician, Huashi Li.
Li has been with the Core for 2 years. She has 4 years of expertise
is in the area of cellular neurobiology.
her master’s degree from the University of Beijing, she
was a research assistant from 2000 to 2006. From 2006 to 2008
she was the laboratory manager for Dr. Gavin Rumbaugh in the
Department of Neurobiology at UAB. Huashi started working in
the Core in 2008 and performs many of the Core services, such
as preparation of neuronal cell cultures and competent cells;
testing and maintenance of equipment and maintains supplies,
training people neuronal primary cell cultures, cell transfections,
and competent cell transformations.
and oversight for the Recombinant Technologies Core are provided
by two faculty members, Drs. Michael Brenner
and Anne Theibert (5% effort), both of whom are Associate Professors
in Neurobiology. Each brings approximately 25 years of experience
in molecular and cellular techniques. Drs. Brenner and Theibert
have a regularly scheduled weekly one-hour meeting with Ms. FLi
and Dr. Phillips to trouble-shoot problems, set priorities for
use of the Core’s resources and consider new services.
In addition, Dr. Brenner is responsible for implementation of
decisions and for assisting Dr. Phillips and Ms. Li on a daily
basis as needed for trouble-shooting problems and administrative