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IDDRC Core-B Resources
Co-Directors: Michael Brenner, Ph.D. and Anne Theibert, Ph.D.
(David Curiel and Tim Townes, Consultants)

Core-B occupies room 571 of the Civitan International Research Center (CIRC) (270 sq feet), room 543 in the Sparks Center (SC) (280 sq feet), and a portion of a shared equipment room in 586 CIRC. Equipment housed in these locations is as follows:

571 CIRC

  • Laptop computer used for core administration.
  • BioRad GenePulser II with a capacitance extender plus and RF module for the introduction of recombinant DNA molecules into mammalian cells and bacteria.
  • Nikon Eclipse Stereoscopic Microscope with an epifluorescence illuminator and digital camera for rapid identification, genotyping and photographing transgenic mouse lines expressing green fluorescent protein (GFP) or stained with X-gal.
  • Tissue Culture Facility that includes two ThermaForma CO2 incubators, a Nuare tissue culture hood, and a Nikon tissue culture microscope.
  • DNA Sequence Analysis Workstation including Vector NTI DNA analysis software and access to the Genetics Computer Group (GCG) – The Wisconsin package which supports comprehensive analysis of sequence information using either Unix based software or the web-based GCG Seqweb. Available analysis tools include sequence comparison, database searching, evolution trees, gene searching, mapping, pattern recognition, primer selection, protein analysis, nucleic acid secondary structure and sequence translation. The Workstation also has a link to the UAB electronic subscription to Current Protocols in Molecular Biology.

586 CIRC

  • UVP-Dual Intensity Transilluminator Plus Photosystem for rapidly obtaining simple hard copy photographs of gels and excising gel fragments
  • UV/Vis Spectrophotometer that can hold standard and mini (50 ?l) quartz cuvettes
  • BioRad gel dryer
  • Savant speed vac

Additional Resources

  • BioRad 170-8720 iCycler™ thermal cycler that can accommodate 0.5 ml tubes, 0.2 ml tubes or 96-well plates for PCR
  • Alpha Innotech 4400 imaging system for gel documentation
    This system provides visualization and quantitation of protein and DNA gels, and of blots developed with visible or fluorescent signals.
  • Tissue culture facility that includes two ThermaForma CO2 incubators, a Nuare tissue culture hood, and a Nikon tissue culture microscope.

In order to conserve Core space, some special equipment under the aegis of the Core is housed in individual laboratories that use them most frequently. These include the following:

  • Transgenomic wave DNA fragment analysis system for SNP nalysis
  • BioRad gene gun for transfecting cells in culture or in tissue slices
  • BioRad two-dimensional gel electrophoresis system
  • Amaxa neucleofector device for high efficiency transfection (>50%) of neurons
  • Small cell microinjector device (Eppendorf micromanipulator and microinjector) for delivering DNA, RNA, proteins (e.g., dominant negative, antibodies), dyes or drugs to specific intracellular locations
  • Oocyte injector, oocyte incubator, and dissection scope

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